Welcome to my logbook and documentation page ;)

My inspiration comes from Joi Ito, the director of the MIT Media Lab. At the Solid Conference 2015 he gave a talk about the future of technology. His statement is ‘Bio is the New Digital’. His quote on which my project it based is: ‘The design of the future is hardware, it’s software, it’s biology and it’s complex.. But you have to know math.’

My goal of the Biohack Academy is to try and tinker with the future of biotechnology myself.

Final presentation

Take a look at my presentation for an update (and pictures): https://docs.google.com/presentation/d/1iQGf3zNRhKPUyp9L-tkgpL7eB5ZujxsHIc1U7SvHuCM/edit?usp=sharing

The documentation of my project is in the week 9 blogpost https://peterjoostennet.github.io/week9/ and week 8 blogpost https://peterjoostennet.github.io/week8/

The design of the future is … biology (part 2)

This is the result of the restriction enzymes project from last week: https://docs.google.com/drawings/d/1U2tj5D8ORotr2cZ_6X7LAENaTFeoE0Trqg2OdQcCXGM/edit?usp=sharing
It is a nice representation of the amount of cutsites in the bacteriophage: https://benchling.com/wpjoosten/f/Rq44S3V1-20190314-synthetic-dna/seq-FbDOARdi-20190314-kopie-lambda/edit There are 7 Hind3 restriction sites, so the digestion should result in 8 fragments. Six are large enough to be seen. EcoR1 should generate 6 fragments, while the Pst1 should give 29 lambda DNA fragments. They are migrated so closely together on the gel that they appear as one band.

The design of the future is … software

One project which is still open is the ordering of synthetic DNA.
The next steps:

• Calculate the primer and PCR mixes
• Multiply the DNA in the PCR
• Do a gel electrophoresis
• Make the lay-out for the exhibition :)